METHOD OF PEPTIDE SYNTHESIS

摘要

A method for synthesising a given peptide or its derivative which contains a proline residue or a proline derivative, at proximity to, or at, the C-terminal end of said peptide is provided. The method comprises a) synthesising on a first resin a C­terminal portion of said peptide, o r its derivative, comprising at least three successive amino acid residues or their derivatives, by successive coupling of selected amino acids, small peptides or their derivatives, b) cleaving the C-terminal portion from said first resin; c) reattaching said C-terminal portion to a second resin which is generally suitable for the synthesis of peptides but is unsuitable for the formation of peptides having a proline residue positioned atthe C-terminal end of said peptide; and d)coupling selected amino acids, small peptides or derivatives to the C-terminal portion.