发明名称 |
METHOD FOR ALBUMIN PURIFICATION |
摘要 |
The present invention is a method of purifying recombinant human serum albumin (rHSA) from a solution, which method comprises to subject a cell culture supernatant (CCS) comprising rHSA cation exchange on a bimodal high salt tolerant matrix; hydrophobic interaction chromatography (HIC); anion exchange; and recovering the purified rHSA. The bimodal high salt tolerant cation exchange matrix used enables performing a purification of a cell culture supernatant directly, in the sense that no further dilution thereof is necessary. Due to its high binding capacity, said bimodal cation exchange matrix also allows use of a smaller amount of matrix as compared to a corresponding conventional cation exchanger matrix. Accordingly, the present invention allows substantial savings as regards volumes and consequently operation costs. |
申请公布号 |
EP1504031(A1) |
申请公布日期 |
2005.02.09 |
申请号 |
EP20030725953 |
申请日期 |
2003.05.09 |
申请人 |
NORTH CHINA PHARMACEUTICAL GROUP CORPORATION;AMERSHAM BIOSCIENCES AB |
发明人 |
BELEW, MAKONNEN;LI, MEI, YAN;ZHANG, WEI |
分类号 |
C07K14/765 |
主分类号 |
C07K14/765 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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