METHODS FOR PARTICLE-ASSISTED POLYNUCLEOTIDE IMMUNIZATION USING A PULSED ELECTRIC FIELD

摘要

Methods are provided for enhancing an immune response induced by administration of a DNA vaccine. In the invention methods a DNA vaccine encoding an antigen and non-chemically associated adjuvant particles are injected into muscle, dermal or mucosal tissue of a subject at substantially the same time and the tissue is subjected to a pulsed electric field of sufficient strength to result in the DNA vaccine entering cells of the target tissue. The immune response to the antigen is enhanced as compared to when the DNA vaccine is administered alone or in combination with either of the electric pulses or the adjuvant particles without the other.