发明名称 |
METHOD FOR THE CONSTRUCTION OF RANDOMIZED GENE SEQUENCE LIBRARIES IN CELLS |
摘要 |
An in vivo method for the construction of randomized gene libraries and/or domain replacement in gene libraries by homologous recombination using a Kluyveromyces lactis killer toxin, in particular the (.gamma.-subunit of the K. lactis killer toxin, as negative selection marker is described. The use o f the (.gamma.-subunit of K. lactis as negative selectable marker increases th e percentage of randomized clones.
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申请公布号 |
CA2521382(A1) |
申请公布日期 |
2004.10.21 |
申请号 |
CA20032521382 |
申请日期 |
2003.04.11 |
申请人 |
ESBATECH AG |
发明人 |
SCHARER-BRODBECK, CLAUDIA |
分类号 |
C07K14/39;C07K16/00;C12N15/00;C12N15/10;C12N15/62;C12N15/65;(IPC1-7):C12N15/62 |
主分类号 |
C07K14/39 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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