发明名称 |
ANTERIOR OCULAR-ASSOCIATED CELL SHEET, THREE-DIMENSIONAL CONSTRUCT AND PROCESS FOR PRODUCING THE SAME |
摘要 |
<p>It is intended to provide an anterior ocular-associated cell sheet or a three-dimensional construct with little structural defects which can be recovered in a state of sustaining cells, the intracellular desmosome structure and basal lamina-like proteins among cells and base material. The anterior ocular-associated cell sheet or the three-dimensional construct can be produced by culturing cells on a cell culture support having a base material surface-coated with a temperature-responsive polymer having an upper limit or lower limit critical dissolution temperature in water of 0 to 80°C, layered the cultured cell layers, if needed, and then: (1) controlling the culture medium temperature to a level higher than the upper limit critical dissolution temperature or lower than the lower limit critical dissolution temperature; (2) if needed, tightly pressing the thus cultured anterior ocular-associated cell sheet or three-dimensional construct to a polymer film; and (3) then peeling off it together with the polymer film.</p> |
申请公布号 |
WO2004070023(A1) |
申请公布日期 |
2004.08.19 |
申请号 |
WO2003JP01248 |
申请日期 |
2003.02.06 |
申请人 |
CELLSEED INC.;YAMATO, MASAYUKI;OKANO, TERUO |
发明人 |
YAMATO, MASAYUKI;OKANO, TERUO |
分类号 |
C12N11/02;A61K35/12;A61L27/38;C12N5/071;(IPC1-7):C12N5/06;A61F9/007 |
主分类号 |
C12N11/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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