CELL EXTRACT OF ESCHERICHIA COLI HAVING MUTATION IN S12 RIBOSOMAL PROTEIN AND PROCESS FOR PRODUCING PROTEIN IN CELL-FREE SYSTEM USING THE SAME

摘要

It is intended to provide an extract, a kit and a method for synthesizing a protein in a cell-free system. The above-described extract is characterized by being prepared from mutant Escherichia coli cells having a mutation in an S1 2 ribosomal protein gene. In a preferred embodiment, the above mutation is suc h a mutation as imparting tolerance to streptomycin or dependence thereon to E . coli whereby the reading efficiency of mRNA codon on ribosome can be improve d and thus the protein productivity can be elevated.