发明名称 |
Nested oligonucleotides containing a hairpin for nucleic acid amplification |
摘要 |
Templates that are engineered to contain a predetermined sequence and a hairpin structure are provided by a nested oligonucleotide extension reaction. The engineered template allows Single Primer Amplification (SPA) to amplify a target sequence within the engineered template. In particularly useful embodiments, the target sequences from the engineered templates are cloned into expression vehicles to provide a library of polypeptides or proteins, such as, for example, an antibody library.
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申请公布号 |
US2004101886(A1) |
申请公布日期 |
2004.05.27 |
申请号 |
US20030628109 |
申请日期 |
2003.07.28 |
申请人 |
BOWDISH KATHERINE S.;FREDERICKSON SHANA;MCWHIRTER JOHN;TOSHIAKI MARUYAMA |
发明人 |
BOWDISH KATHERINE S.;FREDERICKSON SHANA;MCWHIRTER JOHN;TOSHIAKI MARUYAMA |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;C07H21/04;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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