Method for making recombinant proteins

摘要

The present invention relates to scaffold/matrix attachment regions isolated from hamster cells and their use for the industrial production of recombinant proteins and polypep-tides. In one embodiment the invention is related to a method for production of Factor VII polypeptides or Factor VII-related polypeptides comprising a) transfecting a mammalian cell with an expression vector comprising a nucleic acid molecule encoding Factor VII or a Factor VII-related polypeptide, expression control regions operatively linked to thereto and at least one S/MAR element; b) culturing the transfected cell under conditions for expression of Fac-tor VII or a Factor VII-related polypeptide and c) isolating the expressed polypeptide by suit-able means.