发明名称 |
Method of determination of gene function |
摘要 |
The present method is useful for the identification of genes, ORF's and other nucleic acid molecules which are essential for the expression of a specific phenotype in microorganisms. The method employs In vitro transposition in conjunction with an chromosomal integration vector containing a specific gene or genetic element whose function is unknown. Subsequent transformation of a recombination proficient host with the vector and growth first under non-integrating conditions and then under integrating conditions, followed by a selection screen for either single or double crossover events results in transformants that may be subjected to phenotypic screens to determine gene function.
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申请公布号 |
US6673567(B2) |
申请公布日期 |
2004.01.06 |
申请号 |
US20010808658 |
申请日期 |
2001.03.15 |
申请人 |
E. I. DU PONT DE NEMOURS AND COMPANY |
发明人 |
SHARPE PAMELA L.;NAGARAJAN VASANTHA |
分类号 |
C12N15/09;C12N15/10;C12Q1/68;(IPC1-7):C12Q1/00;C12Q1/02;C12Q1/04 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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