METHOD FOR PREPARING CRYSTALLINE STAPHYLOCOCCUS AUREUS DEFORMYLASE AND CRYSTALLINE DEFORMYLASE PREPARED THEREFROM

摘要

PURPOSE: A method for preparing a crystalline Staphylococcus aureus deformylase and a crystalline deformylase prepared therefrom are provided. The deformylase is an important target protein for development of resistant antibiotics, and the crystalline deformylase can be easily X-ray analyzed and has improved degree of crystallinity. CONSTITUTION: A method for preparing a crystalline Staphylococcus aureus deformylase comprises crystallization of Staphylococcus aureus deformylase according to a hanging-drop vapor diffusion method using a reservoir solution containing ammonium sulfate as a precipitating agent, wherein the hanging-drop vapor diffusion method is carried out under the temperature of 18 to 22 deg. C and pH of 10.0 to 10.8; the reservoir solution is a Caps buffer solution; and the reservoir solution contains 0.05 to 0.10 M of Caps buffer solution and 1.9 to 2.1 M of ammonium sulfate.