CRYOPRESERVATION OF HAPTENIZED TUMOR CELLS

摘要

A method of preserving haptenized tumor cells is described. The method emplo ys a freezing medium containing an effective amount of sucrose and human serum albumin in an isotonic buffered saline solution. Cryogenically preserving haptenized cells in such a medium has been found to maintain the integrity o f the tumor cells during storage. The haptenized tumor cells also retain cell- associated antigens and haptens, and are as immunogenic, i.e., capable of inducing immunotherapeutic response, as fresh vaccine in a mouse model of metastatic disease. In a specific embodiment, haptenized cells are exposed t o a solution of 8 % sucrose, 10% human serum albumin in Hank's buffered solution, and then frozen to -80~C overnight and then stored in a liquid nitrogen freezer. Methods of storing haptenized tumor cells and compositions are also provided.