发明名称 |
METHOD FOR DIRECT RESCUE AND AMPLIFICATION OF INTEGRATED VIRUSES FROM CELLULAR DNA OF TISSUES |
摘要 |
A method for isolating AAV viruses from cellular DNA of non-human primate (NHP) tissues by transfecting the DNA of NHP into 293 cells, rescuin g the virus and amplifying it through serial passages in the presence of adenoviru s helper functions is provided. Also provided are kits useful for performing this method.
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申请公布号 |
CA2426283(A1) |
申请公布日期 |
2003.10.29 |
申请号 |
CA20032426283 |
申请日期 |
2003.04.22 |
申请人 |
THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA |
发明人 |
GAO, GUANGPING;ALVIRA, MAURICIO R.;WILSON, JAMES M. |
分类号 |
C12N15/09;C12N7/02;C12N15/35;C12N15/861;C12N15/864;C12P19/34;C12Q1/70;(IPC1-7):C12N7/02;C12N5/10 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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