| 摘要 |
<p>It is intended to provide a reaction solution for cell-free protein synthesis which is suitable for folding a protein having a weakly reduced state, and a cell-free protein synthesis method characterized by performing a weakly reduced synthesis reaction, preferably by adding a substance catalyzing a disulfide bond exchange reaction at the early stage of a translation reaction and then further continuing the translation reaction. According to this method, a protein having a properly formed disulfide bond in its molecule and being comparable in function to the original one can be provided at a high efficiency. More specifically speaking, an antibody protein binding specifically to an antigen is provided.</p> |
