Oral recombinant Lactobacilli vaccines.

摘要

The present invention relates to an oral vaccine comprising recombinant lactic acid bacteria expressing heterologous antigen in vivo intracellularly and/or the surface of the lactic acid bacterium as specific immunogenicity eliciting component for eliciting immunogenicity against the heterologous antigen, characterised in that the recombinant lactic acid bacterium is a Lactobacillus plantarum. Preferably, the recombinant Lactobacillus plantarum comprises an expression vector capable of expressing the heterologous antigen intracellularly and/or such that the heterologous antigen is exposed on the cell surface under conditions present in the gastrointestinal tract. The recombinant Lactobacillus plantarum is preferably a recombinant Lactobacillus plantarum 256. The invention also relates to a recombinant Lactobacillus plantarum, more specifically a recombinant strain of Lactobacillus plantarum 256, for use in the vaccines of the invention; as well as to an expression vector suitable for intracellular expression or exposure of a heterologous antigen encoded thereon, said expression vector providing expression in a Lactobacillus plantarum of the heterologous antigen under conditions existing in the gastrointestinal tract.