| 摘要 |
PROBLEM TO BE SOLVED: To provide antibodies for specifically detecting pathogenic prions for human origin, to provide methods for detecting pathogenic prions, and, in particular, to provide a conformation-dependent immunoassay method for detecting pathogenic prion proteins in a sample of a body fluid, containing a PrP protein. SOLUTION: This conformation-dependent immunoassay method comprises the following steps: (a) adding one of the above-described monoclonal antibodies, which is fixed to a solid support and which exhibits a higher affinity for the first prion protein conformation, to the first portion of the sample, and determining this first concentration, (b) treating the second portion of the sample in order to increase the binding affinity of the second conformation of the prion protein for the monoclonal antibody, (c) adding the monoclonal antibody to the treated second portion of the sample to be investigated, in order to determine the second concentration, and (d) comparing the first prion protein concentration with the second prion protein concentration in order to ascertain the presence of the pathogenic prion protein conformation. COPYRIGHT: (C)2003,JPO
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