| 摘要 |
A computer-based method of performing optical density measurements on a plurality of cell population samples.In the preferred embodiment of the invention, a calibration factor is first established to eliminate the effect of the media used in the wells. Optical density measurements are then made every five minutes on the cell population samples in each of the wells. For measurement of apoptosis, every five minutes it is also necessary to compute for each cell population the slope of the optical density measurement versus time and correct for any effects attributable to the cell populations in the control sample. The values of interest include maximum corrected slope value, the time from initiation of the assay to observance of the maximum optical density value, the time from initiation of the assay to the beginning of apoptosis and the time from the beginning of apoptosis to observance of maximum optical density value.
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