METHOD OF CONSTRUCTING CAMEL ANTIBODY LIBRARY

摘要

It is intended to provide a camel antibody library sustaining the multiplicity in the antibody variable region genes in vivo in animals belonging to the family Camelidae. The multiplicity in the variable region genes in vivo can be achieved by, for example, mixing genes originating in a plural number of individuals or elaborating gene amplification conditions. Although VHH with restricted multiplicity repertory can be merely obtained by known methods, a library containing genes encoding functional VHH in a sufficiently large repertory size can be successfully provided. Thus, a library whereby VHH against an arbitrary antigen can be freely obtained is provided. VHH is excellent in solubility and stability and shows a reactivity which cannot be expected in case of conventional tetramer IgG.