| 摘要 |
An apparatus for quick freezing tissue specimens has the advantage of precise predictable embedding orientation. The apparatus includes embedding wells, chucks and over-chuck cooling blocks. Additional components include a cooled cutting board, freezing plate griddle and elevated freezing block. Tools created to use with the system include a dislodging bar, a spatula/pry tool, a dispensing slide and a flattening tool. The combination of these elements function at -25 to -30 degrees C. to precisely prepare tissue specimens for frozen sectioning. The apparatus can be cooled and adapted by several methods: (1) as a stand-alone bench top cryoembedding station refrigerated by compressor and Peltier devices: (2) as modular portable units cooled in a separate refrigeration device such as a cryostat or freezer; and (3) built into cryostat work chambers as part of the internal embedding center.
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