摘要 |
The present invention relates to a method of quantitative determination of sodium ions in a sample using beta -galactosidase, wherein the beta -galactosidase reaction is conducted in the presence of at least one chelating agent selected from the group consisting of 1,2-cyclohexanediamine-N,N,N',N'-tetraacetic acid, ethylenediamine-tetraacetic acid, triethylenetetramine-hexaacetic acid, diethylenetriamine-N,N,N',N'',N''-pentaacetic acid, 1,3-diaminopropan-2-ol-N,N,N',N'-tetraacetic acid, ethylenediamine-N,N'-dipropionic acid dihydrochloride, ethylenediamine-tetrakis(methylenesulfonic acid), imino-diacetic acid, hydroxyimino-diacetic acid and nitrilo-triacetic acid. The method of quantitative determination of sodium ions of the present invention is useful in clinical examinations and the accuracy in the measurement by the method is high. |