METHOD FOR CONSTRUCTING A CHIMERIC DNA LIBRARY USING A SINGLE STRAND SPECIFIC DNASE

摘要

The method for constructing a chimeric DNA library of the present invention uses matching regions of heterologous DNA sequences as internal primers and enables highly efficient DNA reassembly between heterologous DNAs having relatively low similarity. Therefore, the inventive method can be effectively used for developing a useful gene having better functional property through reassembly between various heterologous DNA sequences of enzyme-encoding gene, promoter, virus and so on.