METHOD OF CONSTRUCTING HOST AND PROCESS FOR PRODUCING FOREIGN PROTEIN

摘要

In a method of constructing an eucaryotic microbial host for producing a foreign protein encoded by a gene which has been transferred by a gene modification method, the production efficiency of the foreign protein in the transformant, wherein the gene encoding the foreign protein has been transferred into a host, is elevated. Under the culture environment of the transformant, the whole genome part unnecessary or harmful to the production of the foreign protein by the transformant is totally or partly deleted or inactivated. Schizosaccharmocyes pombe is favorably employed as the above eucaryotic microbial host. As the genome part of the eucaryotic microbial host to be deleted or inactivated is favorably gene(s) selected from among genes relating to energy metabolism and genes relating to proteases. Specific examples thereof include pyruvate decarboxylase genes, serine protease genes, aminopeptidase genes, carboxypeptidase genes and so on.