KIT FOR DIAGNOSIS OF HEMORRHAGIC FEVER WITH RENAL SYNDROME(HFRS) USING NUCLEOCAPSIDE PROTEIN OF HANTANN VIRUS

摘要

PURPOSE: A kit for diagnosis of hemorrhagic fever with renal syndrome(HFRS) using a nucleocapside protein of Hantann virus is provided, thereby the HFRS can be simply and correctly diagnosed. CONSTITUTION: A baculovirus transfer vector containing a small gene segment of Hantann virus represented by SEQ ID NO: 5 is provided. A recombinant baculovirus(KCTC 0854BP) transformed with the baculovirus transfer vector is also provided. The nucleocapside protein of Hantann virus having the amino acid sequence of SEQ ID NO: 8 is expressed from the recombinant baculovirus(KCTC 0854BP). The kit for diagnosis of hemorrhagic fever with renal syndrome(HFRS) contains the nucleocapside protein of Hantann virus as an antigen, wherein the kit further contains microtiter plate, a buffer solution for analysis, a standard antibody, a second antibody and a chromogenic substrate; the second antibody is combined with chromogenic enzymes or fluorescent materials; and the nucleocapside antigen protein is fixed on a biological microchip.