A UNIVERSAL NUCLEIC ACID AMPLIFICATION SYSTEM FOR NUCLEIC ACIDS IN A SAMPLE

摘要

The invention provides in one embodiment a method for amplifying nucleic acid in a sample comprising providing said sample with a set of primers to enable synthesizing at least one nucleic acid strand complementary to at least part of said nucleic acid, wherein said set of primers comprises between 3-8 random bases preferably clustered near the 3' end of each primer in said set of primers. In a preferred embodiment the method is used for determining whether a sample, derived from a patient, mammal, poultry and fish, comprises nucleic acid of a pathogen. The method is further suited for the typing of said pathogen and the typing of a particular variant of said pathogen. The method is also suited for the elucidation of the gene-expression profile or genetic profile of cells.