| 摘要 |
The acid-labile sub-unit (ALS) of insulin like growth factor binding protein complex in biologically pure form is described.ALS has a molecular weight between 80-115 kd as determined by SDS polyacrylamide gel electrophoresis, run under reducing conditions; and a partial N-terminal amino acid sequence as follows:GlyAspProGlyThrProGlyGluAlaGluGlyProAlaCysProAlaAlaCysAlawherein the first amino acid may be Gly or Ala.Also described are methods of producing ALS, compositions containing the in-vivo IGF protein complex, methods of detecting ALS in body fluids, recombinant nucleic acid sequences encoding ALS, and expression vectors and host cells containing such nucleic acid sequences.
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