Method for modifying and identifying functional sites in proteins

摘要

The invention relates to a method for identifying one or more functional sites in a protein, which is characterized in that a) the target protein is contacted with a binding partner linked to a laser-activatable marker (tag), (BP-tag), to form a complex of target protein and BP-tag, b) the complex of target protein and BP-tag is irradiated with laser light to generate free radicals which selectively alter the bound target protein at the binding site, and c) the selectively altered region of the protein is identified by a combination of protein cleavage and mass spectrometry. The invention further relates to an apparatus for carrying out the method according to the invention.