METHOD FOR PRODUCING PROTEIN

摘要

PROBLEM TO BE SOLVED: To provide an effective method for producing a recombinant protein using Escherichia coli and a technique useful therefor. SOLUTION: A DNA cassette comprising a first promoter DNA derived from a 1ac promoter in a pUC 19 plasmid, a spacer region DNA and a second promoter DNA derived from a promoter region in a neutral protease in Bacillus amyloliquefaciens wherein these are sequentially linked in series in a 5' to 3' direction is prepared and is used as a promoter for expressing a recombinant protein in Escherichia coli.