发明名称 |
Method for maintaining High-quality focus during high-throughput, microscopic digital montage imaging |
摘要 |
Today's technology allows montage images of large areas to be captured, stored and displayed at the resolution limit of the microscope optics. The invention reduces the overall time to capture a microscope slide by reducing the overhead associated with refocusing the optics at each tile location. Using a macroscopic image of the region to be scanned, representative focus positions are selected based on a predefined set of image characteristics. Prior to montage scanning, these focus positions are placed under the microscope optics and a best-focus position determined. A surface is fit to the resulting three-dimensional data. The parameters that define this surface are feed into the scanning control component to allow high-quality focused images to be acquired throughout the scanning process, eliminating the required stop, refocus, acquire image processing steps used in traditional montage imaging systems.
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申请公布号 |
US2002114497(A1) |
申请公布日期 |
2002.08.22 |
申请号 |
US20010788666 |
申请日期 |
2001.02.21 |
申请人 |
WETZEL ARTHUR W.;GILBERTSON JOHN R.;BECKSTEAD JEFFREY A.;FEINEIGLE PATRICIA A.;HAUSER CHRISTOPHER R.;PALMIERI FRANK A. |
发明人 |
WETZEL ARTHUR W.;GILBERTSON JOHN R.;BECKSTEAD JEFFREY A.;FEINEIGLE PATRICIA A.;HAUSER CHRISTOPHER R.;PALMIERI FRANK A. |
分类号 |
G02B21/24;G06T7/00;(IPC1-7):G06K9/00 |
主分类号 |
G02B21/24 |
代理机构 |
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代理人 |
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地址 |
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