| 摘要 |
Apparatus is provided for the detection of chemiluminescence, e.g., the luminescent assay of an analyte in a sample. Sample, namely the production of a permanent photographic record of such chemiluminescence, e.g., by the use of a POLAROID film. The apparatus includes three interrelated elements. The first element comprises a film holder for holding a photographic film sensitive to a chemiluminescent reaction of the sample, the film holder defining a window in registry with any photographic film which may be held in the film holder. The second element comprises a sample holder for holding the sample in registry with the window and any photographic film which may be held in the film holder, and for allowing movements of the sample relative to any photographic film which may be held in the film holder between (i) a raised position, wherein the sample holder, and thus the sample is in spaced relation to any photographic film which may be held in the film holder and (ii) a lowered position, wherein said sample holder is in sufficiently close proximity to any photogarphic film which may be held in the film holder means to allow the sample held thereby directly to contact any photographic film which may be held in the film holder. The third element comprises a shutter operatively associated with the window of the film holder and movable between a closed position, wherein the shutter obstructs the window so as to prevent the sample holder from moving into the lowered position, and an opened position, wherein the window is unobstructed by the shutter so as to permit the sample holder to move from the raised position to the lowered position. The production of the permanent photographic record begins when the test sample in the container is placed in direct contact with the photographic film. The production of the photographic record is stopped when the test sample in the container is removed out of direct contact with the photographic film and the slidig shutter is closed. Various test results can be observed by varying the time of direct contact of the test sample in the reactant container with the photographic film. This provides maximal analytical sensitivity. During the entire test, exposure to ambient light is prevented.
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