| 摘要 |
A method of detecting transmissible spongiform encephalopathies is described which involves (a) treating a sample of tissue, blood or a blood derivative from a test subject with alcohol and a detergent, (b) adding an agent which degrades normal prion protein (c) adding an agent which denatures abnormal prion protein, (d) adding a prion-specific antibody and (e) detecting bindin g of the antibody to the sample. Also described is a method of fixing abnormal prion protein to a substrate comprising (a) treating the protein or a sample suspected to contain the abnormal prion protein with alcohol and an anionic detergent and (b) adding a protease, in the presence of the substrate.</SDOA B>
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