NOVEL COMPOUND SEPARATED FROM AGASTACHE RUGOSA, WITH APOPTOSIS INHIBITING ACTIVITY, PREPARATION METHOD THEREOF, PHARMACEUTICAL COMPOSITION CONTAINING THE COMPOUND, AND USE THEREOF

摘要

PURPOSE: A novel compound and its pharmaceutically acceptable salt separated from Agastache rugosa, its preparation method and a pharmaceutical composition containing the compound and/or the salt are provided, which compound is used as an apoptosis inhibitor. CONSTITUTION: The novel compound is represented by the formula 1 or 2. In the formulas 1 and 2, R1, R2, R3, R4 and R5 are independent each another and are an alkylalkoxy group of C1-C4; H; a straight or branched alkyl group of C1-C6; OH; a dialkylamino group of C2-C6; a straight or branched hydroxyalkyl group of C1-C6; a straight or branched dihydroxyalkyl group of C3-C6; an alkoxyalkyl group of C3-C6; or a saturated or unsaturated five or six-membered hetero ring containing 1-3 hetero atoms selected from N, O and S and unsubstituted or substituted with an alkyl group of C1-C3. The method comprises the steps of extracting Agastache rugosa with an organic solvent 1, concentrating the extract under reduced pressure and extracting the concentrated one with an organic solvent 2 to obtain the extract; concentrating the extract, dissolving the concentrated one into a small quantity of a solvent mixture, and obtaining the concentrated active fraction by chromatography; dissolving the concentrated fraction into an organic solvent 3 and obtaining an active fraction by chromatography; and separating the active fraction by chromatography of the obtained fraction, removing the solvent and lyophilizing it. Preferably the organic solvents 1 and 3 are selected from the group consisting of methanol, ethanol, acetone and distilled water; the organic solvent 2 is selected from the group consisting of ethyl acetate, hexane and butanol; and the solvent mixture is a mixture of chloroform and methanol or hexane and ethyl acetate.