发明名称 |
METHOD FOR PRODUCING ACTIVE SERINE PROTEASES AND INACTIVE VARIANTS |
摘要 |
A method for producing biologically active serine proteases, isolated serine protease domains and their amino acid variants in a prokaryotic host is disclosed. The method comprises an N-terminal addition of a helper sequence consisting of a dipeptide which is suitable for degradation by a dipeptidyl amino peptidase, by the expression of the serine protease(s) and/or its (their) fragments containing the N-terminal dipeptide helper sequence, optionally as inclusion bodies, and by the renaturation of the expressed proteins and the activation of the serine protease(s) and/or serine protease domains by splitting off the helper sequence using an exopeptidase. |
申请公布号 |
EP1214428(A1) |
申请公布日期 |
2002.06.19 |
申请号 |
EP20000964151 |
申请日期 |
2000.09.08 |
申请人 |
MAX-PLANCK-GESELLSCHAFT E.V.;JENNE, DIETER |
发明人 |
JENNE, DIETER;WILHARM, ELKE;PARRY, MARINA, A., A.;BODE, WOLFRAM;HUBER, ROBERT |
分类号 |
C12N15/09;A61K38/48;C12N1/21;C12N9/48;C12N9/64;C12N15/57;C12P21/06 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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