Method for assembly of multiple DNA fragments

摘要

Methods for assembling two or more DNA fragments are disclosed including providing for each of the DNA fragments at least one protruding terminus or overhang capable of hydrogen bonding to a complementary sequence on at least one strand of a second DNA fragment, the overhang and the complementary sequence having at least 15 bases, and mixing two or more of the DNA fragments under conditions suitable to promote joining thereof. DNA constructs are also disclosed which are produced by joining two or more DNA fragments comprising at least one protruding terminus overhang capable of hydrogen bonding to a complementary sequence on at least one strand of a second DNA fragment.