发明名称 |
E.COLI PRODUCED IMMUNOGLOBULIN CONSTRUCTS |
摘要 |
A method of producing recombinant heterotetrameric immunoglobulin from a procaryotic organism which includes providing a procaryotic organism that has been transformed with DNA encoding the heavy and light chains of an immunoglobulin having a binding site for immunologically binding a preselected antigen and an amino acid sequence which signals the export of the immunoglobulin from the cytoplasm of the organism, the DNA being operationally associated with a promoter recognizable by RNA polymerase endogenous to the organism, and culturing the transformed procaryote for a time and under conditions sufficient to allow the organism to export the immunoglobulin from the cytoplasm, wherein the exported heterotetrameric immunoglobulin retains its native conformation and binding specificity for the preselected antigen.
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申请公布号 |
US2002037558(A1) |
申请公布日期 |
2002.03.28 |
申请号 |
US19910781395 |
申请日期 |
1991.10.23 |
申请人 |
LO KIN-MING;GILLIES STEPHEN D. |
发明人 |
LO KIN-MING;GILLIES STEPHEN D. |
分类号 |
C07K14/245;C07K16/18;C12N15/70;C12P21/08;(IPC1-7):C12Q1/68;C12P21/06;C12P21/04;A61K39/395;C12N1/00;C12N1/20;C07K16/00 |
主分类号 |
C07K14/245 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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