METHOD FOR DETECTING HEPATITIS C VIRUS WITH HYBRIDOMAS

摘要

A method of directly detecting Hepatitis C virus in a fractionated or non-fractionated serum of a patient, which comprises detecting said virus with primers corresponding to viral RNA encoding core protein which said RNA is a light fraction of the total viral RNA, the said light fraction being isolated after ultracentrifugation in a CsCl gradient of human serum containing HCV virus, said light fraction containing most of the circulating infectious HCV virus particles characterized by precipitating RNA, then effecting reverse transcription, and then effecting amplification with the primers described herein.