| 发明名称 |
METHOD OF AFFINITY PURIFYING PROTEINS USING MODIFIED BIS-ARSENICAL FLUORESCEIN |
| 摘要 |
The present invention features methods for purifying polypeptides of interest using a modified Fluorescein arsenical helix binder (F1AsH) compound immobilized on a solid support. An exemplary F1AsH target sequence motif is also presented. Examples of modification of the F1AsH compound which allow immobilization to a solid support are also provided. The present invention also provides DNA constructs for producing a dual affinity tagged polypeptide and methods for purification thereof.
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| 申请公布号 |
WO0153325(A3) |
申请公布日期 |
2002.03.07 |
| 申请号 |
WO2001US02214 |
申请日期 |
2001.01.22 |
| 申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
VALE, RONALD, D.;THORN, KURT;COOKE, ROGER;MATUSKA, MARIJA;NABER, NARIMAN |
| 分类号 |
C07F9/80;C07K1/22;C07K19/00;(IPC1-7):C07D305/00;C07D307/00;C07D309/00;C07D313/00;C07D315/00;C07D317/00;C07D319/00;C07D323/00;C07D325/00;C07D329/00;C07D407/00;C07D493/00;C07C49/213;C07C49/225;C07C49/563;C07C49/76;C07F213/00 |
主分类号 |
C07F9/80 |
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