发明名称 |
MARINE BACTERIUM PSEUDOMONAS AERUGINOSA BYK-2 PRODUCING RHAMNOLIPID AND METHYL RHAMNOLIPID SIMULTANEOUSLY |
摘要 |
PURPOSE: Provided is a marine bacterium Pseudomonas aeruginosa BYK-2 which produces simultaneously rhamnolipid and methyl rhamnolipid used for the remediation of an oil spill. CONSTITUTION: The isolation method of Pseudomonas aeruginosa BYK-2 comprises the steps of: shacking-culturing a seawater sample in an isolation medium of a microbial strain for 7 days then followed by inoculating the medium to sterilized media and shacking-culturing them for 3 days; selecting a medium showing high oil decomposability then smearing it on a modified seawater medium to isolated a single strain; inoculating it to sterilized media then shacking-culturing them for 7 days; selecting a medium having excellent oil decomposability again then smearing it on a seawater medium to a single strain, Pseudomonas aeruginosa BYK-2. Where, shacking-culture is performed at 25 deg.C and 180rpm.
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申请公布号 |
KR20020016707(A) |
申请公布日期 |
2002.03.06 |
申请号 |
KR20000049891 |
申请日期 |
2000.08.26 |
申请人 |
KONG, JAE YEOL |
发明人 |
HA, SUN DEUK;KIM, BONG JO;KIM, HAK JU |
分类号 |
C12N1/00;(IPC1-7):C12N1/00 |
主分类号 |
C12N1/00 |
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