发明名称 |
Method and test kit for analyzing DNA repair |
摘要 |
A method for analyzing the repair of DNA modifications and base mispairings as well as apurinic and apyrimidinic sites by DNA repair enzymes, comprising the following steps: contacting (a) single- or double-stranded DNA molecules which were covalently coupled to a solid-phase matrix carrying primary or secondary amino groups by reaction with a reactive squaric acid derivative, and which have modifications and/or base mispairings and/or apurinic or apyrimidinic sites, with (b) a composition containing DNA repair enzymes; and determining the elimination of the DNA modifications and/or base mispairings and/or apurinic or apyrimidinic sites. The DNA molecules are covalently coupled to the solid state matrix via a primary or secondary amino group incorporated in the DNA molecule at the 5'-end or at the 3'-end of the DNA or in the 2'-position of at least one deoxyribosyl residue.
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申请公布号 |
US2002022228(A1) |
申请公布日期 |
2002.02.21 |
申请号 |
US20010848116 |
申请日期 |
2001.04.30 |
申请人 |
NEHLS PETER;GLUSENKAMP KARL HEINZ |
发明人 |
NEHLS PETER;GLUSENKAMP KARL HEINZ |
分类号 |
C12N15/09;C07K16/44;C12Q1/68;(IPC1-7):C12Q1/68;C07H21/04 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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