发明名称 |
ETO1 AND RELATED PROTEINS, AND METHODS OF REGULATING ETHYLENE BIOSYNTHESIS |
摘要 |
The invention provides a negative regulator of ethylene biosynthesis in plants, which is embodied by <i>ETHYLENE-OVERPRODUCER1 (ETO1)</i>, a recessive gene, which controls the expression of the synthesis of the plant hormone ethylene. <i>ETO1</i> is a member of a novel gene family, which is highly conserved in the plant kingdom, and which is unique in that it comprises both BTB and TPR domains. Two-hybrid experiments, together with <i>in vitro</i> binding experiments and double mutant analyses <i>in planta</i> revealed direct interaction of ETO1 with 1-aminocyclopropane-1-carboxylate synthase (ACS), a key enzyme of ethylene biosynthesis. Furthermore, functional assay in <i>E. coli</i> showed that members of the related ETO1-LIKE (EOL) proteins also significantly inhibit ethylene biosynthesis. Thus, the invention further provides methods of controlling, regulating and modulating ethylene biosynthesis in plants, plant cells, and the like.
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申请公布号 |
WO0157063(A8) |
申请公布日期 |
2002.02.07 |
申请号 |
WO2001US03994 |
申请日期 |
2001.02.07 |
申请人 |
THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA;ECKER, JOSEPH, R.;YOSHIDA, HITOSHI;WANG, LONG-CHI;LURIN, CLAIRE |
发明人 |
ECKER, JOSEPH, R.;YOSHIDA, HITOSHI;WANG, LONG-CHI;LURIN, CLAIRE |
分类号 |
C07K14/415;C12N15/29;C12N15/82;(IPC1-7):C07H21/04;C07K14/00;C07K16/16 |
主分类号 |
C07K14/415 |
代理机构 |
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地址 |
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