METHOD OF MEASURING TOTAL HOMOCYSTEINE

摘要

A method of quickly, conveniently and highly sensitively detecting and quantifying homocysteine which comprises oxidizing, in the presence of an SH reagent, remaining homocysteine accessory substrates, the formed homocysteine converting enzyme product or the enzyme reaction products thereof to thereby form hydrogen peroxide, and measuring the thus formed hydrogen peroxide via color-development with the use of an oxidative color-developing agent; and a kit therefor. By using the above method and kit, homocysteine in biological samples (in particular, bodily fluids such as blood and urine) can be quickly, conveniently and highly sensitively detected and quantified.