| 摘要 |
PURPOSE: A process for producing beta-secretase is provided, thereby mass-producing the beta-secretase which is necessary for the study on Alzheimer's disease. CONSTITUTION: The process for producing beta-secretase comprises the steps of: selectively amplifying cDNA of beta-secretase and inserting the beta-secretase gene into a vector to produce a recombinant expression vector; transforming a host cell with the recombinant expression vector; cultivating the transformed host cell; and separating and purifying the beta-secretase produced, in which the beta-secretase gene is normal or mutant gene(D215N), wherein 215th amino acid D(Asp) is substituted by N(Asn); the transformed host cell can be E. coli BL21(DE3)/pET22b-BACE22501 (KCTC 18031P), E. coli BL21(DE3)/pET19b-MBACE46419 (KCTC 18040P), and mammal HEK293 CELL/pcDNA3-BACE (KCLRF-BP-00031).
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