摘要 |
A method of testing sperm quality comprising obtaining a sample of sperm to be tested; detecting and measuring amount of testis-specific HspA2 chaperone protein in the sperm; and determining a sperm quality parameter based upon the amount of chaperone protein, wherein an increased amount of the chaperone protein indicates a higher sperm quality. The chaperone protein is detected and measured either by binding one or more antibodies specific to the sperm chaperone protein to the sperm and measuring the antibody content or measuring ATP bound to the sperm chaperone protein. In the case of the latter method, the chaperone protein may be detected and measured by measuring the ATP bound to the sperm chaperone protein, and such measuring is by chaperone protein-bound and CK-B generated ATP measurement, or by chaperone protein ATP bioluminescence.
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