| 摘要 |
<p>Assay for urinary protein and creatinine is effected using a reaction vessel, comprising: (a) a horizontal axis of rotation and includes a reaction channel contg. first and second reaction zones; (b) a sample delivery device with a sample inlet, and (c) a viewing port between the reaction zones, where (c.1)the first reaction zone contains a dried antibody, (c.2) the second reaction zone contains a dried Li salt (I) of an organic acid, and a dried alkaline material (II), and (c.3) the sample can be transported between the reaction zones by gravity when the vessel is rotated about its axis. The assay comprises: (a) introducing a urine sample through the delivery device and introducing a reaction fluid contg. a polymeric agglutinating agent, which specifically binds with the antibody in the first reaction zone to solubilise the antibody and cause an agglutination reaction which can be quantified by taking readings through the viewing port; and (b) rotating the vessel to bring the sample and reaction fluid into contact with (I) and (II), so that (I) and (II) are dissolved and the pH is raised to 11.5-12.5, resulting in a colour reaction between the organic acid anion and any creatinine in the sample, and determining the extent of the colour reaction.</p> |
