| 摘要 |
PROBLEM TO BE SOLVED: To provide a human type anti-TNFαantibody and to provide a method for producing the same. SOLUTION: The H chain polypeptide of a recombinant antibody against human TNFα, having at least one of amino acid sequences: Gly-Tyr-Thr-Phe- Thr-Asn-Tyr-Gly-Met-Asn as CDR-H1, Trp-Ile-Asn-Thr-Tyr-Thr-Gly-Glu-Pro-Thr- Tyr-Ala-Asp-Asp-Phe-Lys-Gly as CDR-H2, and Tyr-Asp-Tyr-Asp-Gly-Phe-Asp-Tyr as CDR-H3, or its fragment. The L chain polypeptide of a recombinant antibody against human TNFα, having at least one of amino acid sequences: Thr-Ala-Ser- Ser-Ser-Val-Ser-Phe-Ser-Tyr-Leu-His as CDR-L1, Tyr-Ser-Thr-Ser-Asn-Leu-Ala- Ser as CDR-L2, and His-Gln-Tyr-Leu-Arg-Ser-Pro-Tyr-Thr as CDR-L3. The human type antibody or its fragment against human TNFα, comprising the H chain polypeptide or its fragment and the L chain polypeptide. The method for producing the human type anti-TNFα antibody, comprising transforming a host cell with an expression vector having a gene encoding the antibody or the like and then culturing the transformant. |
