| 摘要 |
An optical detection system is adapted for detection of biological reactions. An excitation source illuminates a portion of an object to be examined, the portion preferably comprising one microlocation out of an array of microlocations. An intervening optical detection platform serves to direct the excitation radiation to the portion of the object to be illuminated. A detector receives the emitted radiation from the object to be examined, the detector being characterized in that the diameter of the region examined by the detector is the same as or smaller than the diameter of the illuminated region, and comprises less than the entire surface of the object to be examined, and most preferably images a whole or a part of a single microlocation. In operation, a microscopy system is formed in which the excitation radiation is substantially in focus at the surface of the object to be examined. In one aspect of this invention, the optical detection platform includes an excitation detector that measures reflected excitation radiation from the object to be examined. This information is compared to prestored information regarding the location of the microlocations and interstitial regions on the object to be examined, whereby alignment information is obtained. The excitation radiation may then be precisely directed to a given microlocation or portion thereof to perform the examining through the system. |
