| 摘要 |
The invention relates to a method of detecting and identifying nucleic aicd (NA) molecules within a population (I) of NA molecules of identical, similar or different sequence, in which: (a) the single-stranded or duplex ribonucleic acid (RNA) molecules or single-stranded deoxyribonucleic aicd (DNA) molecules contained in a population (I) iare convereted into a population (II) of duplex DNA molecules; b) the population (II) of duplex DNA molecules is subjected to denaturation and subsequent renaturation, so that a mixture of homo- and heteroduplex DNA molecules is present; c) heteroduplexes formed in the population (II) where unpaired of incorrectly paired nucleotides are present are reacted chemically with a carbodiimide compound; and d) the resulting carboddimide reactions products are characterized and quantified, characterized in that a chromatographic purification method is carried out between each of the steps a to d.
|
