发明名称 |
Mammlian two-hybrid system |
摘要 |
The invention provides a method of using a reporter gene that encodes a fluorescent polypeptide to indicate that an interaction has occurred between a bait and a prey protein in a mammalian cell. An advantage of using a fluorescent reporter polypeptide is that an interaction between a bait and prey in a mammalian cell can be readily detected, e.g., within 96 hours. In another method of the invention, a prey plasmid contains an Epstein-Barr virus origin of replication (ori-P). The OriP permits the prey plasmid to replicate episomally and indefinitely without damaging the mammalian cell or integrating into the genomic DNA of the mammalian cell. Since such a plasmid is maintained episomally in a circular form, it can be readily recovered from the mammalian cell.
|
申请公布号 |
US2001024794(A1) |
申请公布日期 |
2001.09.27 |
申请号 |
US20010876614 |
申请日期 |
2001.06.07 |
申请人 |
THE GENERAL HOSPITAL CORPORATION, MASSACHUSETTS CORPORATION |
发明人 |
SHIODA TOSHIHIRO;ISSELBACHER KURT J. |
分类号 |
C12N15/10;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/10 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|