发明名称 |
METHOD OF CLEAVING SINGLE-STRANDED RNA AND KIT |
摘要 |
A method of site-specifically and efficiently cleaving a single-stranded RNA. This cleaving method, which comprises hybridizing a target RNA with an antisense oligonucleotide 1 complementary to the 5'-end domain of the target part of the target RNA and another antisense oligonucleotide 2 complementary to the 3'-end domain of the target part of the target RNA to give a nucleic acid complex and then bringing this nucleic acid complex into contact with an ion, etc. capable of cleaving a single-stranded RNA, is characterized in that intercalaters are introduced into the 5'-end of the antisense oligonucleotide 1 and/or the 3'-end of the antisense oligonucleotide 2, the nucleic acid complex has a gap and a desired cleavage site exists in the gap. A kit usable in effecting this method and a gene therapy method are also provided.
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申请公布号 |
WO0168832(A1) |
申请公布日期 |
2001.09.20 |
申请号 |
WO2001JP01590 |
申请日期 |
2001.03.01 |
申请人 |
KOMIYAMA, MAKOTO;KUZUYA, AKINORI |
发明人 |
KOMIYAMA, MAKOTO;KUZUYA, AKINORI |
分类号 |
A61K48/00;C12N15/113;(IPC1-7):C12N15/01;C12N15/11 |
主分类号 |
A61K48/00 |
代理机构 |
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地址 |
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