发明名称 |
EXPRESSION AND SECRETION VECTOR FOR HUMAN INTERFERON ALPHA AND PROCESS FOR PRODUCING HUMAN INTERFERON ALPHA BY EMPLOYING SAME |
摘要 |
Disclosed in this invention are: an expression vector for the secretive production of human interferon alpha (hIFN.alpha.) comprising a polynucleoti de encoding a modified E. coli thermostable enterotoxin II signal sequence and a polynucleotide encoding hIFN.alpha.) ligated to the 3'-end thereof; a microorganism transformed with the expression vector; and a process for secretively producing human interferon by culturing the microorganism, said process being capable of secreting a soluble form of active hIFN.alpha.), which does not contain an additional methionine residue at its N-terminal, into the periplasm of an E. coli cell.
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申请公布号 |
CA2397527(A1) |
申请公布日期 |
2001.08.09 |
申请号 |
CA20012397527 |
申请日期 |
2001.01.19 |
申请人 |
HANMI PHARM. CO. LTD. |
发明人 |
JUNG, SUNG YOUB;CHOI, KI DOO;KWON, SE CHANG;KIM, CHA SOON;BAE, SUNG MIN;LEE, GWAN SUN |
分类号 |
C12N15/09;C07K14/245;C07K14/56;C12N1/21;C12N15/21;C12N15/63;C12N15/70;C12P21/02;(IPC1-7):C12N15/21 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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