| 摘要 |
The invention relates to a method for sequencing in parallel at least two different nucleic acids present in a nucleic acid mixture, characterized in that (a) a surface is provided, which surface possesses islands of nucleic acids of in each case the same type, i.e. tertiary nucleic acids; (b) counterstrands of the tertiary nucleic acids, i.e. TNCs, are provided; (c) the TNCs are extended by one nucleotide, with the nucleotide at the 2'-OH position or at the 3'-OH position carrying a protecting group which prevents further extension, the nucleotide carrying a molecular group which enables the nucleotide to be identified; (d) the incorporated nucleotide is identified; (e) the protecting group is removed and the molecular group of the incorporated nucleotide, which is used for identification, is removed or altered, and (f) step (c) and subsequent steps are repeated until the desired sequence information has been obtained. |
