| 摘要 |
A novel growth medium called "H15" is disclosed that supports much higher E. coli cell densities and, concomitantly, a much higher yield of plasmid than previously reported for small-scale applications. The high-yield growth medium contains two buffers with different pKa values and an additional nucleotide source. On a unit volume basis, E. coli cultures grown in this medium consistently produce 5-10 times, and sometimes up to 30 times, more recombinant plasmid than in conventional rich media, paralleling the increase in cell density. This phenomenon is independent of E. coli host strain, DNA insert size and plasmid copy number. H15 medium is economical and high yields can be achieved using standard research laboratory equipment.
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